Papers – Dengue & Arbovirosis


An overview of mosquito vectors of Zika virus.
Boyer S, Calvez E, Chouin-Carneiro T, Diallo D, Failloux AB.
Microbes Infect. 2018 Mar 2. pii: S1286-4579(18)30039-X. doi: 10.1016/j.micinf.2018.01.006. 


Dengue-1 virus and vector competence of Aedes aegypti (Diptera: Culicidae) populations from New Caledonia.
Calvez E, Guillaumot L, Girault D, Richard V, O’Connor O, Paoaafaite T, Teurlai M, Pocquet N, Cao-Lormeau VM, Dupont-Rouzeyrol M.
Parasit Vectors. 2017 Aug 9;10(1):381. doi: 10.1186/s13071-017-2319-x.

A neutralization assay for Zika and Dengue viruses using a real-time PCR-based endpoint assessment
Wilson HL, Tran T, Druce J, Dupont-Rouzeyrol M, Catton M.
J Clin Microbiol. 2017 pii: JCM.00673-17. doi: 10.1128/JCM.00673-17.

Socioeconomic and environmental determinants of dengue transmission in an urban setting: an ecological study in Nouméa, New Caledonia.
Zellweger M, Cano J,  Mangeas M, Taglioni F, Mercier A, Despinoy M, Menkès C, Dupont-Rouzeyrol M, Nikolay B and Teurlai M. 
PLoS Negl Trop Dis. 2017 11(4):e0005471.

Zika virus infection and myasthenia gravis: Report of 2 cases.
Molko N, Simon O, Guyon D, Biron A, Dupont-Rouzeyrol M, Gourinat AC.
Neurology. 2017 Feb 10. pii: 10.1212


Vertical Transmission of Dengue Virus in the Peripartum Period and Viral Kinetics in Newborns and Breast Milk: New Data.
Arragain L*, Dupont-Rouzeyrol M*, O’Connor O, Sigur N, Grangeon JP, Huguon E, Dechanet C, Cazorla C, Gourinat AC, Descloux E.
J Pediatric Infect Dis Soc. 2016. pii: piw058.

Global spread of chikungunya virus a lesson for Aedes-transmitted arboviruses.
Duarte dos Santos C, Dupont-Rouzeyrol M, Sam IC, Roques P. CAB
Reviews. 2016 11(18):1-12.

 Zika virus infection as an unexpected finding in a Leptospirosis patient.
Biron A, Cazorla C, Amar J, Pfannstiel A, Dupont-Rouzeyrol M, Goarant C.
JMM Case Rep. 2016 3. doi: 10.1099

Infectious Zika viral particles in breastmilk
Dupont-Rouzeyrol M, Biron A, O’Connor O, Huguon E, Descloux E. .
Lancet. 2016 Mar 12;387(10023):1056.

Differential Susceptibilities of Aedes aegypti and Aedes albopictus from the Americas to Zika Virus
Chouin-Carneiro T, Vega-Rua A, Vazeille M, Yebakima A, Girod R, Goindin D, Dupont-Rouzeyrol M, Lourenço-de-Oliveira R, Failloux AB.
PLoS Negl Trop Dis. 2016 Mar 3;10(3):e0004543.

Whole-Genome Sequencing Analysis from the Chikungunya Virus Caribbean Outbreak Reveals Novel Evolutionary Genomic Elements.
Stapleford KA, Moratorio G, Henningsson R, Chen R, Matheus S, Enfissi A, Weissglas-Volkov D, Isakov O, Blanc H, Mounce BC, Dupont-Rouzeyrol M, Shomron N, Weaver S, Fontes M, Rousset D, Vignuzzi M.  PLoS Negl Trop Dis. 2016 Jan 25;10(1):e0004402.

Genetic Diversity and Phylogeny of Aedes aegypti, the Main Arbovirus Vector in the Pacific.
Calvez E, Guillaumot L, Millet L, Marie J, Bossin H, Rama V, Faamoe A, Kilama S, Teurlai M, Mathieu-Daudé F, Dupont-Rouzeyrol M. 
PLoS Negl Trop Dis. 2016 Jan 22;10(1):e0004374.


Co-infection with Zika and Dengue Viruses in 2 Patients, New Caledonia, 2014.
Dupont-Rouzeyrol M, O’Connor O, Calvez E, Daurès M, John M, Grangeon JP, Gourinat AC.
Emerg Infect Dis. 2015. 21(2):381-2.

Detection of zika virus in urine.
Gourinat AC*, O’Connor O*, Calvez E, Goarant C, Dupont-Rouzeyrol M. 
Emerg Infect Dis. 2015. 21(1):84-6.


Epidemiological and molecular features of dengue virus type-1 in New Caledonia, South Pacific, 2001-2013.
Dupont-Rouzeyrol M, Aubry M, O’Connor O, Roche C, Gourinat AC, Guigon A, Pyke A, Grangeon JP, Nilles E, Chanteau S, Aaskov J, Cao-Lormeau VM. 
Virol J. 2014. 31;11:61.

The emm-cluster typing system for group A Streptococcus identifies epidemiologic similarities across the Pacific region
Baroux N, D’Ortenzio E, Amédéo N, BakerC, Alsuwayyid BA, Dupont-Rouzeyrol M, O’Connor O, Steer A, Smeesters P.
Clin Infect Dis. 2014. 59(7):e84-92.

Isolation and partial characterization of bacteria (Pseudoalteromonas sp.) with potential antibacterial activity from a marine costal environment from New Caledonia.
Dufourcq R, Chalkiadakis E, Fauchon M, Deslandes E, Kerjean V, Chanteau S, Petit E, Guezennec J, Dupont-Rouzeyrol M. 
Lett Appl Microbiol. 2014. 58(2):102-8.


Breast milk as a possible route of vertical transmission of dengue virus?
Barthel A, Gourinat AC, Cazorla C, Joubert C, Dupont-Rouzeyrol M, Descloux E. 
Clin Infect Dis. 2013 Aug;57(3):415-7.

Partial characterization of an exopolysaccharide secreted by a marine bacterium, Vibrio neocaledonicus sp. nov., from New Caledonia.
Chalkiadakis E, Dufourcq R, Schmitt S, Brandily C, Kervarec N, Coatanea D, Amir H, Loubersac L, Chanteau S, Guezennec J, Dupont-Rouzeyrol M, Simon-Colin C.
J Appl Microbiol. 2013 Jun;114(6):1702-12.


Chikungunya virus and the mosquito vector Aedes aegypti in New Caledonia (South Pacific Region).
Dupont-Rouzeyrol M, Caro V, Guillaumot L, Vazeille M, D’Ortenzio E, Thiberge JM, Baroux N, Gourinat AC, Grandadam M, Failloux AB.
Vector Borne Zoonotic Dis. 2012. 12(12):1036-41.

Use of serum and blood samples on filter paper to improve the surveillance of dengue in Pacific Island Countries.
Aubry M, Roche C, Dupont-Rouzeyrol M, Aaskov J, Viallon J, Marfel M, Lalita P, Elbourne-Duituturaga S, Chanteau S, Musso D, Pavlin BI, Harrison D, Kool JL, Cao-Lormeau VM.
J Clin Virol. 2012 Sep;55(1):23-9.

Lower respiratory infections among hospitalized children in New Caledonia: a pilot study for the Pneumonia Etiology Research for Child Health project.
Mermond S, Zurawski V, D’Ortenzio E, Driscoll AJ, DeLuca AN, Deloria-Knoll M, Moïsi JC, Murdoch DR, Missotte I, Besson-Leaud L, Chevalier C, Debarnot V, Feray F, Noireterre S, Duparc B, Fresnais F, O’Connor O, Dupont-Rouzeyrol M, Levine OS. 
Clin Infect Dis. 2012 Apr;54 Suppl 2:S180-9.


Aetiology of community-acquired pneumonia in hospitalized adult patients in New Caledonia.
Mermond S, Berlioz-Arthaud A, Estivals M, Baumann F, Levenes H, Martin PM.
Trop Med Int Health. 2010 Dec;15(12):1517-24.


Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae.
Page S, Vernel-Pauillac F, O’Connor O, Bremont S, Charavay F, Courvalin P, Goarant C, Le Hello S.  Antimicrob Agents Chemother. 2008 Nov;52(11):4155-8.

Regional Course about Leptospirosis

2017_lorcRegional course  about Leptospirosis

held from 13 to 17 November 2017

Opening session by Mrs. Valentine Eurisouké, member of government in charge of health.






Follow up with:

For more information:

Leptospira biodiversity

An unexpected Leptospira biodiversity in New Caledonian soils
Principal investigator Cyrille Goarant
Focal point IPNC Roman Thibeaux, Cyrille Goarant
Collaborators at IPNC Marie-Estelle Soupé-Gilbert, Dominique Girault, Emilie Bierque
Other Collaborators Mathieu Picardeau, IPP, Gregorio Iraola and Ignacio Ferres, IP Montevideo
Total budget of project 3000 € Budget devoted to IPNC : NA
Funding IPNC
Timeline Start date: 2016 End date: 2018

Following the demonstration of the environmental survival of pathogenic leptospires at sites of human contamination in New Caledonia, isolation of Leptospira were undertaken.

Infectious strains were not re-isolated from the environment, but a large number of isolates were obtained. Difficulties of identification (MALDI-TOF, 16S rRNA sequencing) led us to suspect novel species and to undertake the sequencing of their complete genome.

Perform a genomic study of leptospires isolated from Caledonian soils.

Isolations were carried out from field soil or water samples. Liquid cultures in EMJH medium were supplemented with a combination of antibiotics described to select growth of leptospires (Chakraborty et al, 2011). The cultures were then spread on EMJH agar medium for isolation.

The isolates were identified with MALDI-TOF and by sequencing their complete genome. Strains of the pathogenic and intermediate clusters are tested in animal models.

More than 100 environmental isolates have been obtained. The first 26 strains for which the complete genome was sequenced showed the presence of 12 novel species: 3 in the pathogen cluster, 5 intermediates and 4 saprophytes.

Strains of the pathogen cluster were unable to cause acute infection in hamsters or renal carriage in mice.

A detailed comparison of core genomes and accessory genomes supports the hypothesis of a polyphyletic group of leptospires with low virulence within the pathogen cluster. This result suggests, in terms of evolution, that virulence was acquired independently in the pathogen and intermediate clusters.

Preliminary results from other isolates suggest other new species.



These 12 novel species should be validly described and named. The new isolates will also be studied. Specific PCR will be designed and tested.
Vincent AT, Schiettekatte O, Goarant C, Neela VK, Bernet E, Thibeaux R, Ismail N, Mohd Khalid MK, Amran F, Masuzawa T, Nakao R, Amara Khorba A, Bourhy P, Veyrier FJ, Picardeau M, 2019. Revisiting the taxonomy and evolution of pathogenicity of the genus Leptospira through the prism of genomics. PLoS Negl Trop Dis 13: e0007270.

Thibeaux R, Iraola G, Ferrés I, Bierque E, Girault D, Soupé-Gilbert ME, Picardeau M, Goarant C, 2018. Deciphering the unexplored Leptospira diversity from soils uncovers genomic evolution to virulence. Microbial Genomics 4: 000144.

Thibeaux R, Girault D, Bierque E, Soupé-Gilbert ME, Rettinger A, Douyere A, Meyer M, Iraola G, Picardeau M, Goarant C, 2018. Biodiversity of environmental Leptospira: improving identification and revisiting the diagnosis. Front. Microbiol. 9: 816.

Thibeaux R, Iraola G, Ferrés I, Bierque E, Girault D, Soupé-Gilbert ME, Picardeau M, Goarant C. Of soils, Leptospira and humans. Présentation orale à la conférence de l’ILS, Palmerston North, NZ, November 2017.